Journal: Journal of clinical pathology

Article Title: Immunohistochemical panel to differentiate endometrial stromal sarcoma, uterine leiomyosarcoma and leiomyoma: something old and something new

doi: 10.1136/jclinpath-2015-202915

Figure Lengend Snippet: Conditions and titrations of the 10 antibodies

Article Snippet: ASC1 , Novus , 1:100 , PH 8.0 20 min , liver.

Techniques: Control

Journal: Journal of clinical pathology

Article Title: Immunohistochemical panel to differentiate endometrial stromal sarcoma, uterine leiomyosarcoma and leiomyoma: something old and something new

doi: 10.1136/jclinpath-2015-202915

Figure Lengend Snippet: Expressions of the 10 antibodies in 94 cases of leiomyoma, ESS and ULMS

Article Snippet: ASC1 , Novus , 1:100 , PH 8.0 20 min , liver.

Techniques:

Journal: Journal of clinical pathology

Article Title: Immunohistochemical panel to differentiate endometrial stromal sarcoma, uterine leiomyosarcoma and leiomyoma: something old and something new

doi: 10.1136/jclinpath-2015-202915

Figure Lengend Snippet: Positive expression of ASC1 in (A) low grade (LG) endometrial stromal sarcoma (ESS), (B) ‘LG’ uterine leiomyosarcoma (ULMS), (C) leiomyoma (LM). ASC1 was strongly expressed in cytoplasmic and nuclear patterns. Positive expression of stathmin1 in (D) in ESS, (E) leiomyosarcoma and (F) LM. Stathmin1 was strongly expressed in cytoplasmic and nuclear patterns.

Article Snippet: ASC1 , Novus , 1:100 , PH 8.0 20 min , liver.

Techniques: Expressing

Journal: Journal of clinical pathology

Article Title: Immunohistochemical panel to differentiate endometrial stromal sarcoma, uterine leiomyosarcoma and leiomyoma: something old and something new

doi: 10.1136/jclinpath-2015-202915

Figure Lengend Snippet: Comparison of biomarker expressions between all ESS and ULMS

Article Snippet: ASC1 , Novus , 1:100 , PH 8.0 20 min , liver.

Techniques: Comparison, Biomarker Discovery

Journal: Journal of clinical pathology

Article Title: Immunohistochemical panel to differentiate endometrial stromal sarcoma, uterine leiomyosarcoma and leiomyoma: something old and something new

doi: 10.1136/jclinpath-2015-202915

Figure Lengend Snippet: Comparison of biomarker expressions between LG ESS and ‘LG’ ULMS

Article Snippet: ASC1 , Novus , 1:100 , PH 8.0 20 min , liver.

Techniques: Comparison, Biomarker Discovery

Journal: Cancers

Article Title: N-Acetylcysteine Promotes Metastatic Spread of Melanoma in Mice

doi: 10.3390/cancers14153614

Figure Lengend Snippet: Effect of inhibition of Cys/cystine uptake or GSH synthesis on GSH levels and metastatic activity in B16-F10 cells. Cancer cells were transfected in vitro, as explained under Material and Methods, before their in vivo inoculation. B16-F10 cells were inoculated i.v. (portal vein). NAC (240 mg/kg) was administered orally for 10 days, starting 120 min after B16-F10 cells inoculation. All measurements were performed in the liver or in metastatic cells isolated from the liver 10 days after tumor inoculation. Results obtained using melanoma cells treated with scrambled RNA sequences were not significantly different from those displayed as non-treated controls. * Significantly different p < 0.01, comparing shRNA-treated cells versus controls; + Significantly different p < 0.01, comparing NAC-treated mice versus mice treated with vehicle (n = 9–10).

Article Snippet: Knockdown of SLC7A10 (codes the ASC1 Na + -independent neutral amino acid transporter) was performed using an ASC1 shRNA Plasmid sc-39159-SH from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Inhibition, Activity Assay, Transfection, In Vitro, In Vivo, Isolation, shRNA, Control

Journal: Nature communications

Article Title: Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex.

doi: 10.1038/s41467-023-37528-3

Figure Lengend Snippet: Fig. 2 | Interactions of TRIP4 variants in cells. a Western blots (WB) monitoring immunoprecipitation (IP) of ASCC1, ASCC2, and ASCC3 by the indicated N-terminally Flag-tagged TRIP4 variants from the cell extracts. b Western blots (WB) monitoring immunoprecipitation (IP) of ASCC3 by the indicated HA-tagged

Article Snippet: Western blotting was performed using antibodies against the Flag tag (Sigma-Aldrich F3165; 1:7500), ASCC1 (Proteintech 12301-1-AP; 1:500), ASCC2 (Proteintech 11529-1-AP; 1:1000) andASCC3 (Proteintech 17627- 1-AP; 1:1000).

Techniques: Western Blot, Immunoprecipitation

Journal: Nature communications

Article Title: Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex.

doi: 10.1038/s41467-023-37528-3

Figure Lengend Snippet: Fig. 2 | Interactions of TRIP4 variants in cells. a Western blots (WB) monitoring immunoprecipitation (IP) of ASCC1, ASCC2, and ASCC3 by the indicated N-terminally Flag-tagged TRIP4 variants from the cell extracts. b Western blots (WB) monitoring immunoprecipitation (IP) of ASCC3 by the indicated HA-tagged

Article Snippet: Western blotting was performed using antibodies against the Flag tag (Sigma-Aldrich F3165; 1:7500), ASCC1 (Proteintech 12301-1-AP; 1:500), ASCC2 (Proteintech 11529-1-AP; 1:1000) andASCC3 (Proteintech 17627- 1-AP; 1:1000).

Techniques: Western Blot, Immunoprecipitation

Journal: bioRxiv

Article Title: Serine Racemase is a Cysteine Racemase and Physiologic Down Regulator of Insulin Promoter Methylation

doi: 10.1101/2022.05.17.492243

Figure Lengend Snippet: ( A ) HEK293 cells were transfected with ASCT2 and treated with different concentrations of D- cysteine (x axis) for 30 minutes at 37°C. Cells were harvested, washed and lysed and D-cysteine levels measured using luciferase assay. Data show levels of intracellular D- cysteine indicated by luminescence (y axis). ( B ) HEK293 cells were transfected with Asc-1 and treated with exogenous D-cysteine for 30 minutes at 37°C. Cells were harvested, washed and lysed and D-cysteine concentration measured using luciferase assay. Data show levels of intracellular D-cysteine indicated by luminescence. ( C ) Litter mate WT, Asc1 +/- and Asc1 -/- pups (10 day old) pancreas were harvested. Samples were pooled homogenized and sonicated to extract free endogenous D-cysteine and supernatants assayed using luciferase assay. Luminescence was measured in the supernatant samples and D-cysteine concentrations obtained from standard curve. Each genotype had N=10-12 mice. Data are representative of 3 independent experiments. ( D ) Pancreatic and liver lysates of WT and age-matched Asc1 -/- pups (litter mates) showing expression of SR. Actin was a loading control. (E) Schematic of D- cysteine transport in HEK293 cells by ASCT2 and Asc1 transporters.

Article Snippet: D-cysteine transport experiments were performed in HEK293 cells transiently transfected with human SLC1A15 (ASCT2; Origene; Cat# RC200305) and rat SLC7A10 (Asc1; Genomics-online.com; Cat# ABIN4047848) cDNA using lipofectamine 3000 reagent.

Techniques: Transfection, Luciferase, Concentration Assay, Sonication, Expressing

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: Summary of the 15 antibodies used for the mass cytometry analysis.

Article Snippet: Anti-Human CD49c , 161Dy , Fluidigm , 3161016B , .

Techniques: Mass Cytometry